This research paper evaluates the antibacterial activities of plant extract from Heliotropium zeylanicum against six pathogenic bacteria by disc diffusion method. The ethanol plant extract exhibited significant antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and moderate activity against other test organisms. The chloroform extract showed moderate activity against E.coli and Bacillus subtilis. The aqueous extract was not proved as a potential source for antibacterial activity