Present work intends to study the effect of physical activity on blood glucose levels among the urban adults of Tirupati town, Chittoor district, Andhra Pradesh. A simple random sampling technique is employed in selecting the subjects. Structured and standardized questionnaire is used to collect the data on the demography, SES, physical activity, habit of smoking and alcohol, lifestyles, anthropometry and fasting blood glucose levels in 802 adults (421 males; 381 females) with an age range of 30-70 years. Secondary and higher level of education is more in both genders. 12 percent of the sample is habituated to sedentary physical activity. Sex differences are persisted for anthropometry, blood glucose and systolic blood pressure (p<0.05). Sedentary activity is positively correlated with body mass index (F=12.54; p<0.05) and blood glucose in males (F=6.75; p<0.05). Similarly subjects involved in sedentary physical activity are characterized by elevated frequencies of obesity and hyperglycemics. OR for moderate physical activity is 4.464 times (95% CI: 1.128-17.664) higher when compared to heavy physical activity subjects. Moderate activity subjects are 7.145 times (95% CI: 2.129-23.980) at risk in the elevation of blood glucose levels. The risk of elevatied in blood glucose levels decreased to 4.013 times (95% CI: 1.205-13.359) in mild activity subjects. In conclusion it is inferred that involving in moderate or heavy physical activity may reduce the risk of developing type-2 diabetes.