Infection associated with metallo-β-lactamase (MBL) producing organisms particularly Pseudomonas aeruginosa is associated with higher mortality and morbidity rates and health care costs. The main objective of this study was to assess the prevalence susceptibility profiles of Metallo lactamases producing strains among multidrug resistant Pseudomonas aeruginosa isolated from various clinical samples. A total of 60 multidrug resistant Pseudomonas aeruginosa strains were tested for a period of January 2012 to August 2012. Antimicrobial susceptibility testing, determination of MIC, and detection of MBL producing P.aeruginosa strains were performed as per CLSI guidelines. Among the 60 isolates tested 20 (33.3%) isolates were positive for MBL production. P.aeruginosa showed highest percentage of sensitivity to colistin and next to Polymixin B. MBL production was detected by both combined disc (CD) method and MIC assay. All MBL isolates were multidrug resistant. Majority of MBL producers were from urine and pus. MBL-producing P.aeruginosa were detected from various clinical samples in this study and this data can be used as base-line information of this novel type of β-lactamase in our setup