Abstract
COMPARING SOME METHODS OF ENTERIC VIRUS CONCENTRATION FROM WATER SAMPLES

Human enteric viruses are water-borne infectious agents that can be detected appropriately only if an effective virus concentration method is applied. often the adsorption-elution principle is used. We considered making a comparative study of some primary concentration methods as well as two secondary virus concentration methods. To evaluate the efficacy of the methods RT-PCR using various dilution of Oral Poliovirus Vaccine (OPV) was done. The results showed that the adsorption / elution on cellulose acetate filter is more effective than on silica gel or floated gauze. For secondary concentration the use of PEG 8000 gave better results than organic flocculation.