We determine the mineral composition and lave evaluate the vasorelaxant activity of the powders and extracts of Lippia multiflora from 4 zones of Benin: Kétou, Mono, Djidja and Savalou. The main minerals found in the leaves are sodium (Na), potassium (K), magnesium (Mg), calcium (Ca). The Lippia multiflora samples in the four Kétou, Savalou, Mono and Djidja regions have calcium contents of 2800.7624mg / 100g, 2200.6034mg/ 100g, 2800.6240 mg/100g and 2800.1238mg/100g, respectively. These values are higher than the limit recommended by the WHO (800mg / 100g / day). The potassium content of the samples is respectively 700.8545mg / 100g, 1600.5812mg / 100g, 700.1855 mg / 100g and 1100, 7123 mg / 100g. What is below the 2000mg / 100g / day limit. For magnesium, the amount in the samples is 504 mg / 100g, 307 mg / 100g, 326 mg / 100g, and 510 mg / 100g respectively, compared with a normal value of 350mg / 100g for adults. The sodium content of the samples is 4150.5014mg / 100g, 2270.2344 mg / 100g, 2570.6953mg / 100g and 2610.9309mg / 100g, respectively, for a recommended value of 500mg / 100g / day. The aqueous decoction of Lippia multiflora of Djidja gave a good relaxation on the coronary arteries at a concentration of 25.81mg / mL compared to the extracts of the other zones. In total, the aqueous decoction of Lippia multiflora from Djidja is the most active followed by that of the Mono sample with a concentration of 21.32 mg / mL. The vasorelaxant activity observed for the Djidja sample is endothelium-dependent and the vasoactive factor NO (nitric oxide) is involved in this relaxation.