Department of Medical Parasitology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.

Department of Medical Parasitology, Faculty of Medical Laboratory Sciences, Al Neelain University, Khartoum, Sudan.
Plasmodium falciparum ,Genotype ,Merozoite Surface Protein ,

Genotyping of Plasmodium falciparum based on PCR amplification of the polymorphic genes encoding the merozoite surface proteins 1 and 2 (msp1 and msp2) is well established in the field of malaria research to determine the number and types of concurrent clones in an infection. Genotyping is regarded essential in anti-malarial drug trials to define treatment outcome, by distinguishing recrudescent parasites from new infections. MSP-1 is the major surface antigen of merozoites and is the best-studied protein. During the invasion process, several proteins of the 195-KDa MSP-1 are shed, leaving a highly conserved 19 KDa C-terminal processing fragment (MSP-19) that contains epitopes targeted by antibodies that inhibit erythrocytic invasion. MSP-2 is a second merozoite surface antigen. It is smaller polypeptide with a molecular weight of 45 KDa that is processed during parasite maturation. The antibody response is directed almost completely towards variant regions of MSP-2. The conserved regions are rarely recognized. All samples screened for P.falciparum msp-1 alles, msp-2 alles by PCR. The results revealed that the overall Plasmodium falciparum detection rate between MSP-1&-2, PCR based genotyping of msp-1 allele showed that the rate of msp-2 was higher (53%) than msp-1(13%). Conclusion: We conclude that our finding might probably be attributed to strain differences, and or ethnic differences.

4 , 11 , 2014