ISSN

2277 - 3282

e ISSN

2277 - 3290

Publisher

Journal of Science

NEUROLOGICAL PATHOGENS CHARACTERIZATION BY MOLECULAR AMPLIFICATION TECHNIQUES-CLINICAL RELEVANCE FOR THE DISEASE MANAGEMENT
Author / Afflication
Suneel Kumar Sachan

Sai Institute of Paramedical & Allied Sciences, Dehradun, U.K.
Aayushi Shukla

Sai Institute of Paramedical & Allied Sciences, Dehradun, U.K.
Narotam Sharma

Central Molecular Research Laboratory, Department of Biochemistry, SGRR Institute of Medical & Health Sciences, Patel Nagar, Dehradun, India
Indu Bushan

Sai Institute of Paramedical & Allied Sciences, Dehradun, U.K.
Rinky Rai

Sai Institute of Paramedical & Allied Sciences, Dehradun, U.K.
Aanchal Nehra

Sai Institute of Paramedical & Allied Sciences, Dehradun, U.K.
Smrati Yadav

Sai Institute of Paramedical & Allied Sciences, Dehradun, U.K.
Renu Nautiyal

Sai Institute of Paramedical & Allied Sciences, Dehradun, U.K.
R.K. Singh

Central Molecular Research Laboratory, Department of Biochemistry, SGRR Institute of Medical & Health Sciences, Patel Nagar, Dehradun, India
Keywords
Encephalitis ,Meningitis ,CNS disorders ,Brain biopsy ,Enteroviruses ,Nested PCR ,
Abstract

Pathogens responsible for meningitis, encephalitis and Neurological related disorders are of clinical significance. Herpes Simplex Virus type 1 and type 2 and Mycobacterium tuberculosis complex are few examples. The current study involved genotyping of Herpes Simplex Virus type 1 and type 2 and characterization of Mycobacterium tuberculosis complex in CSF by molecular methods. 32 CSF specimens from patients with clinical suspicion of viral meningitis and 58 from patients suspected for bacterial meningitis was considered for the molecular characterization of Mycobacterium tuberculosis complex. Among 32 patients, suspected for viral meningitis, 03 cases were HSV type 1 positive, where as 5 cases were with both HSV type 1&2. Among the 58, strongly suspected for Tuberculosis meningitis, 20 (34.4%) were TB PCR positive with 12 (60 %) Male and 8 (40 %) female and 38 cases(65.5 %) were negative. It was seen Adenosine Deaminase was elevated in 30 cases but 28 cases was having less than 10 IU/L. Correct, novel, significant molecular diagnostic tools are very important for all those laboratories performing routine diagnosis of tuberculosis in PCR based laboratories settings.

Volume / Issue / Year

5 , 2 , 2015

Starting Page No / Endling Page No

81 - 85