Faculty of Medical Laboratory Sciences, El-Neelain University, Khartoum, Sudan.

Faculty of Medical Laboratory Sciences, El-Neelain University, Khartoum, Sudan.

Department of Biology and Biotechnologies, Faculty of Science and Technology, Al Neelain University, Sudan.

Faculty of Medical Laboratory Sciences, El-Neelain University, Khartoum, Sudan.
Merowe dam , Northern State ,Sudan ,

Merowe dam is located in the Northern State of Sudan across the River Nile. The dam is a hydroelectric and irrigation scheme that may probably develop climatic and environmental changes which might have profound impact on the vectors and vector borne diseases in the area not only by the echo changes but also through the new labors and settlers. The study aimed also to study the distribution of each Plasmodium species in the population of the area and to investigate the possibility of the presence of Wucheraria bancrofti prevalence in the mosquitoes and population as well. A total of 260 volunteers (64.2% of them were females; age ranged between 10 and 92 years) were selected by systematic random sampling method. Demographic and socioeconomic data were obtained using predesigned structured questionnaire. Blood samples were taken on filter papers for screening of Plasmodium spp and Wucheraria bancrofti DNA using real time PCR. The analysis of the genomic DNAs obtained from 260 healthy volunteers, were positive for Plasmodium spp in 135 cases (51.9%). Of these, P. falciparum spp was seen in 121 (89.9%), while P. vivax in only 2 cases (1.5%), and mixed infection was seen in 12cases (8.9%).The PCR analysis showed positive Wucheraria bancrofti in 3 cases out of 260. Plasmodium falciparum is the predominant species detected in the study population, P.vivax has been identified for the first time including mixed infection with P. falciparum in 8.9% of the study population. Wucheraria bancrofti has been detected for the first time in both human subjects and Culex mosquito.

5 , 6 , 2015