ISSN

2277 - 3282

e ISSN

2277 - 3290

Publisher

Journal of Science

AGE-DEPENDENT EXPRESSION OF MUC4 AND MUC13 GENE IN SUTAI (DUROC×MEISHAN) PIGLETS
Author / Afflication
Wenhua Dong

College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China.
Qiufang Zong

College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China.
Lina Gan

College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China.
Weiyun Qin

College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China.
Guoqiang Zhu

College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China.
Wenbin Bao

College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, Yangzhou University, Yangzhou 225009, China.
Shenglong Wu

College of Animal Science and Technology, Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China.
Keywords
MUC4 gene ,MUC13 gene ,mRNA expression ,
Abstract

MUC4 and MUC13 proteins have several biological functions, such as the lubrication, protection of surface epithelium, cell multiplication, immune responses and cell adhesion. Based on Sutai pig, the expression of MUC4 and MUC13 gene were assayed by Real-time PCR to detect the 11 tissue expression and analyzed the differential expression among the different day-age (8-day, 18-day, 30-day and 35-day) . This study aimed at analyzing the expression profile and developmental expression patterns of MUC4 and MUC13 gene in Sutai pigs, in order to provide a theoretical basis for further study of the function and mechanisms of MUC4 and MUC13 gene. RT-PCR analysis showed MUC4 and MUC13 were present in all tissues among all developmental stages. The results revealed higher expression was in 30 and 35-day individuals than that in the 8 and 18-day individuals in 11 tissues, with significant differences in the liver, spleen, stomach, lymphatic, duodenum and jejunum (P < 0.05). MUC13 gene showed the similar expression rule as the MUC4 gene in the liver, spleen, lung, duodenum and jejunum(P < 0.05). The experiment results indicated that MUC4 and MUC13 gene might play a role in protection of surface epithelium, immune response and resisting gastrointestinal infection of pathogenic microorganisms in weaned piglets. MUC13 highly expressed in the duodenum and jejunum tissues and showed obvious developmental expression rule, which may resist infection from pathogenic microorganisms in weaned piglets

Volume / Issue / Year

5 , 11 , 2015

Starting Page No / Endling Page No

1024 - 1028