ISSN

2277 - 3282

e ISSN

2277 - 3290

Publisher

Journal of Science

UTILITY OF IHC MARKERS: HEP PAR 1 AND MOC 31 IN DIFFERENTIATING PRIMARY AND METASTATIC MALIGNANCY OF LIVER IN FINE NEDDLE ASPIRATES
Author / Afflication
Jyoti Chaubey

Jyoti Chaubey
Shipra Singh

Shipra Singh
D. Datta

D. Datta
Mili das

Mili das
Sampriti baruah

Sampriti baruah
Jahanabi Saharia

Jahanabi Saharia
Keywords
FNA of liver, IHC- immunohoistochemistry, HEP PAR 1 AND MOC 31, Cell block. ,
Abstract

The pathological distinction between primary hepatocellular carcinoma (HCC) and metastatic carcinoma(MC) is difficult, especially when diagnosis has to be made on small samples from fine-needle aspiration (FNA). Immunohistochemical studies have been frequently applied to differentiate between them. To evaluate the efficacy of HEP PAR 1 and MOC 31 in differentiating Hepatocellular carcinoma and Metastatic carcinoma on fine-needle aspiration(FNA) samples. 30 paraffin embedded cell blocks representing 11HCC, 19MC(6 from gall bladder, 2 pancreas, 2 lung, 1 stomach, 8 unknown) were immunostained with antibodies for HEP PAR1 and MOC 31. 3 cases were excluded because H&E recuts showed insufficient material for immunohistochemical evaluation(1 poorly differentiated HCC and 2 MC). HEP PAR 1 stained 9 out of 10 HCC cases and 3 out of 17 MC cases. The positivity was cytoplasmic, diffuse, and granular. These 3 MC cases included 2 from lung &1 from stomach. HEP PAR 1 negative HCC was poorly differentiated.MOC 31 stained positively for 15 out of 17 MC while noneof HCC cases were stained positive. The positivity was membranous and (or) cytoplasmic. The two cases negative for MOC 31 were poorly differentiated metastatic adenocarcinoma (MA). The results of the current study demonstrate that HEP PAR1 and MOC 31 are effective markers to differentiate between HCC and MC.Although 3 of the MC cases in the current study were found to be positive with HEP PAR 1, with the help of clinical correlation and other immunohistochemical stains a definite diagnosis could be rendered.For MOC 31 negative cases site-specific markers can also be obtained depending on the clinical scenario.

Volume / Issue / Year

7 , 12 , 2017

Starting Page No / Endling Page No

366 - 371