Frankincense is a hard and woody plant and it is highly un-amicable for enormous and rapid propagation in the field. Frankincense is endemic in Oman and it grows with specific climatic condition and it’s not amenable to grow. Continuous harvesting of this plant for medicinal uses leads to endanger to this particular species. To overcome this issue, first time we establish in vitro plant tissue culture stringency for Omani frankincense. Leaf explants collected from frankincense were cultured on Murashige and Skoog (MS) supplemented with 2,4- dichlorophenoxyacetic acid (2,4- D) and Indole acetic acid (IAA) to induce callus. Data revealed that 2,4-D at 2, 5 and 10 mg/L can induce the callus from leaf explants 83.3%, 83.3%, 71.4% respectively. Whereas, IAA 2 and 10 mg/L induce the callus 84.6%, 84.6% respectively. Further establishment of genetic transformation protocol for Omani frankincense is ongoing in our laboratory