Non-diphtherial Corynebacteria also known as Diphtheroids is being increasingly reported with invasive nosocomial diseases. Little is known about Corynebacteria infection in hospital environment of developing country like India. This prospective study was performed to determine the frequency of different species from clinical sources and the susceptibility profiles of different Corynebacteria isolated from patients of a tertiary care hospital of eastern India. A total of 150 isolates of different Coryneform species were studied here. The isolates were identified to species level by conventional methods and susceptibility profiles were determined. Corynebacterium amycolatum was the predominant species (26.67%) in our series followed by C.pseudodiphtheriticum (14%). More than 35% of the isolates were resistant to oxacillin, clindamycin, penicillin, gentamicin, and ciprofloxacin while all were exclusively sensitive towards vancomycin, linezolid, and tigecycline. Our study showed that Non-diphtherial Corynebacterium species are important cause of nosocomial multidrug resistant infections and continuous efforts to accurate identification at the species level and regular surveillance of their resistant patterns are necessary