Otitis media is major health problem of children and adults in low income countries. This study was done to determine the bacterial etiology and their antimicrobial susceptibility pattern of otitis media. This cross –sectional study included 100 ear swabs collected from patients who clinically diagnosed with otitis media aged between 3 months to 60 years with mean (16.02)years old conducted in Khartoum Ear, Nose and Throat hospital, Khartoum state, Sudan, during August to October 2015. Isolation of pathogens and antimicrobial susceptibility tests were performed using disc diffusion technique as per standard Kirby – Bauer method. out of 100 ear swabs samples, 84(84%) showed bacterial growth. The most frequent isolate were Staphylococcus aureus 29(33.7%), Pseudomonas aureuginosa 24(27.9%), Proteusspp (11.6%), Staphylococcus epidermsdis (10.5%), Citrobacter spp (2.3%) and E.Coli (1.2%). Gentamicin and cholormphenicol have high activity against most of bacterial isolates