Pyogenic infections can lead to severe morbidity and mortality. Though different studies show isolation of same organisms from pus samples yet there is considerable difference in antibiotic susceptibility pattern followed by the organisms. The study is conducted to find out the common organisms in pus samples and their antibiotic sensitivity pattern. The study was conducted in department of Microbiology of Silchar Medical College & Hospital between November, 2015 to April, 2016. Pus samples collected from various clinical departments were processed according to standard microbiological techniques and Antibiotic sensitivity testing is done using Kirby Bauer, s Disc diffusion technique according to C.L.S.I guideline .Out of 248 pus samples received 201 cases (81.05%) showed culture positivity while 47(18.95%) were culture negative. Maximum contribution of pus samples were from Surgery department (59.68%). The most common organism isolated was Staphylococcus aureus (39.3%) followed by Klebsiella species (19.9%), Escherichia coli (16.41%),Pseudomonas (14.42%), Acinetobacter (4.47%),CONS (2.98%) & Proteus (2.48%).Gram positive cocci were 100% sensitive to Vancomycin& Linezolid. Gram negative bacilli showed highest susceptibility to Imipenem & least sensitive to Cefuroxime. Pseudomonas showed maximum resistance towards Ceftazidime. The change in antibiotic susceptibility pattern is a major concern since it may lead to ineffective treatment of pyogenic lesions. Thus appropriate and judicious use of antibiotics should be encouraged to prevent emerging drug resistant organism.