Known as high-molecular-weight proteins, laminins are critical components of the extracellular matrix. These proteins cause cell locomotion, cohesion, and differentiation. Additionally, it affects the growth of the embryo and the healing of wounds. Objectives: An assessment of histological changes during skeletal muscle healing is the purpose of this study. Methods: In this study, In order to mimic skeletal muscle injury, 55 male rabbits' Extensor digitorum longus fibers were used and 3&4 weeks after injury, it was examined. Three groups of animals were examined: control (A) without injuries, group (B) at the fourth after-injury week, and group (C) at the seventh after-injury week. In addition to histological analysis, Laminin antibodies were used for immunohistochemistry. Quantitative analysis of immunohisto chemical reactivity is carried out using Aperio image scope software. Both cellular morphological changes and degeneration overlapping occurred during the regeneration process. A new myotube formed during the fourth week along with the appearance of myoblast like cells. By the end of the seventh week following surgery, the formation of muscular fascicles and muscular histologic developments were visible.Results: With the aid of Aperio softwear, a quantitative analysis of laminin revealed that the 4th-week subgroup had a value of 0.730 * 0.02 pixels while the 7th-week subgroup had a value of 0. The 4th-week group had a level of 0.730 * 0.02 pixels, according to Aperio Soft Wear, whereas the 7th-week group had a value of 0. P-value = 0.0001 indicates that there was a substantial difference between the 326 * 0.03 pixel group and the control group. Conclusion: Degeneration and rejuvenation are linked in the regeneration process