laboratoire de biologie et cytology animales UFR/SN Université Nangui Abrogoua, 02 BP 802 Abidjan 02 Côte d’Ivoire.

Laboratoire de Virologie, Microbiologieet Qualité/Ecotoxicologie & Biodiversité (LBMQ/ETB) Faculté des Sciences et Techniques Mohammedia (FSTM) Université Hassan II Mohammedia-Casablanca Morocco

Laboratoire de Virologie, Microbiologieet Qualité/Ecotoxicologie & Biodiversité (LBMQ/ETB) Faculté des Sciences et Techniques Mohammedia (FSTM) Université Hassan II Mohammedia-Casablanca Morocco
Virus Concentration , Enteric Viruses , Enteric Viruses ,

Human enteric viruses are water-borne infectious agents that can be detected appropriately only if an effective virus concentration method is applied. often the adsorption-elution principle is used. We considered making a comparative study of some primary concentration methods as well as two secondary virus concentration methods. To evaluate the efficacy of the methods RT-PCR using various dilution of Oral Poliovirus Vaccine (OPV) was done. The results showed that the adsorption / elution on cellulose acetate filter is more effective than on silica gel or floated gauze. For secondary concentration the use of PEG 8000 gave better results than organic flocculation.

4 , 3 , 2014