572UNDERSTANDING THE NATIVE POLYACRYLAMIDE SLAB GEL ELECTROPHORESIS PATTERN OF SERUM LDH ISOENZYMES IN CLINICAL SAMPLESThe present study evaluates the electrophoretogram of serum lactate dehydrogenase for understanding the isoenzymes pattern under different clinical conditions. Serum samples were procured from 25 well-matched controls and 25 clinically approved patients. The clinical conditions comprise acute myocardial infarction, chronic kidney disease, hepatic cirrhosis, pulmonary emphysema and infiltrating ductal carcinoma of breast. The lactate dehydrogenase isoenzyme separation was carried out using lab-made native polyacrylamide slab gel electrophoresis. As documented in previous studies, the electrophoretogram of lactate dehydrogenase isozyme in case of controls exhibited the pattern as LDH2>LDH1>LDH3>LDH4≤LDH5. The classical flipped pattern of acute myocardial infarction was also apparent in our study. In all the remaining clinical conditions, relatively dominant LDH5 zone was observed. In addition to LDH5, in pulmonary emphysema, LDH3 was also dominant coexisting zone. In certain cases of AMI and CKD, abnormal electrophoretogram with isomorphic pattern was observed. Isomorphic pattern was more dominant in AMI. The isomorphic pattern with dominant LDH5 was the distinct feature of CKD. Hence, this study further supports the efficacy of native PAGE in understanding the LDH isoenzyme pattern in normal and pathological conditions.chowdarymvp@gmail.comResearch752017 Lactate dehydrogenase (LDH),Polyacrylamide gel electrophoresis ,Acute myocardial infarction ,Chronic kidney disease, Hepatic cirrhosis, Pulmonary emphysemaB Sangeetha,MVP Chowdary Department of Biochemistry, Karpagam Faculty of Medical Sciences & Research, Coimbatore, Tamilnadu, India., Department of Biochemistry, Karpagam Faculty of Medical Sciences & Research, Coimbatore, Tamilnadu, India.