61ANTIMICROBIAL ANALYSIS OF LEAVES OF CANNABIS SATIVAThe leaves of Cannabis sativa belonging to the family Cannabaceae were screened for their antimicrobial activity against Escherichia coli, Staphylococcus aureus, Streptococcus pneumoniae and Salmonella typhii using agar well diffusion method. Methanol extract showed larger inhibition zone against Salmonella typhii as compared to Escherichia coli, Streptococcus pneumoniae and Staphylococcus aureus.It showed highest inhibition zone of 22mm. Ethanol extract showed larger inhibition zone against Streptococcus pneumoniae as compared to Escherichia coli, Staphylococcus aureus and Salmonella typhii. It showed highest inhibition zone of 19mm. Acetone extract showed larger inhibition Salmonella typhii zone against as compared to Staphylococcus aureus, Escherichia coli and Streptococcus pneumoniae. It showed inhibition zone of 29mm. Water extract showed largest inhibition zone against Streptococcus pneumoniae as compared to Salmonella typhii, Escherichia coli and Staphylococcus aureus. Phytochemicals like saponins, tanins, quinones, alkaloids were found present in the plant extracts.monika187@rediffmail.comResearch422014Cannabis sativa,Antimicrobial,Herbal Extract,PhytochemicalsMonika,Naveenth Kour,Mandeep KaurDepartment of Biotechnology, Mata Gujri College, Fatehgarh Sahib-140406, Punjab, India,Department of Biotechnology, Mata Gujri College, Fatehgarh Sahib-140406, Punjab, India,Department of Biotechnology, Mata Gujri College, Fatehgarh Sahib-140406, Punjab, India